This report provides a description of the research activities completed during the CERF Marine Biodiversity Hub survey of southeast Tasmanian temperate reefs, aboard RV Challenger, as part of the Hub's Surrogates Program. The survey was undertaken as a collaborative exercise between the Tasmania Aquaculture and Fisheries Institute (TAFI, University of Tasmania) and Geoscience Australia (GA), and was completed in two stages during 2008 and 2009. This report describes the methods employed in the mapping and video characterisation of shallow-shelf temperate reef habitats across seven survey sites in southeast Tasmania. Preliminary results are provided of the analysis of multibeam sonar and underwater video data. Examples of the types of biota encountered in the towed video and stills photography, and initial interpretations of the benthic communities are also provided. In addition, initial results are presented from the deployment of an Autonomous Underwater Vehicle (AUV) to collect high resolution photographs of reefs and associated biota.

This dataset contains species identifications of crinoids collected during survey SOL4934 (R.V. Solander, 27 August - 24 September, 2009). Animals were collected from the Joseph Bonaparte Gulf with a benthic sled. Specimens were lodged at Museum of Victoria on the 19 April 2010. Species-level identifications were undertaken by Kate Naughton at the Museum of Victoria and were delivered to Geoscience Australia in December 2010. See GA Record 2010/09 for further details on survey methods and specimen acquisition. Data is presented here exactly as delivered by the taxonomist, and Geoscience Australia is unable to verify the accuracy of the taxonomic identifications.

High-precision measurements of N2 in benthic chamber waters indicated that denitrification occurs within the major sedimentary facies in Port Phillip Bay. The integrated fluxes of biogenic N2 , ammonia, nitrate and nitrite showed that the stoichiometric relationship between organic C and N in the muddy sediments, occupying about 70% of the seafloor, was 5.7, this being similar to the Redfield ratio of 6.6. High denitrifying efficiencies (75-85%; denitrification rates ~1.3 mmol N2 m-2 day-1) at organic carbon loadings of ~15-25 mmol m-2 day-1 indicate that most N processed through the sediments was returned to the overlying waters as biologically (generally) unavailable N2. At sites of high organic carbon loadings to the sediments (>100 mmol m-2 day-1) denitrification rates and denitrifying efficiencies were near zero and most N is returned to the Bay waters as biologically available ammonium. In chambers 'spiked' with 15NO3 , denitrifyers used nitrate produced in the sediments in situ, rather than the exogenous nitrate in overlying waters. The sedimentary microbial processes of ammonification, nitrification and denitrification are therefore tightly coupled.

Acorn worms (Enteropneusta), which were previously thought to be a missing link in understanding the evolution of chordates, are an unusual and potentially important component of many deep-sea benthic environments, particularly for nutrient cycling. However very little is known about their distribution, abundance, or behaviour in deep-sea environments around the world, and almost nothing is known about their distribution within Australian waters. In this study, we take advantage of two large-scale deep-sea mapping surveys along the eastern and western continental margins of Australia to quantify the distribution, abundance and trail-forming behaviour of this highly unusual taxon. This is the first study to quantify the abundance and trail behaviour of acorn worms within Australian waters and provides the first evidence of strong depth-related distributions.

Australia is increasingly recognised as a global hotspot for sponge biodiversity, with sponges playing key roles in habitat provision, water quality, bioerosion, and biodiscovery. Despite the intense focus on marine resource management in northern Australia, there is a large knowledge gap about sponge communities in this region. This study focuses on shelf environments of the Timor Sea, in particular the Van Diemen Rise and Londonderry Rise which are characterised by extensive carbonate terraces, banks and reefs, separated by soft sediment plains and deeply incised valleys. These carbonate terraces and banks are recognised as a Key Ecological Feature (KEF) in the marine region plans for northern Australia (North and Northwest Marine Regions) and are in part incorporated into the Oceanic Shoals Commonwealth Marine Reserve. To support the management of this marine reserve and its associated KEF, we use new datasets to investigate regional patterns in sponge assemblages and their relationships to seabed geomorphology. To do this, we use sponge assemblage data and multibeam-derived variables (depth, backscatter, slope, geomorphic feature) from seven survey areas located on the Van Diemen Rise (four sites) and Londonderry Rise (three sites), spanning approximately 320 km in an east-west direction. The dataset was collected during three collaborative surveys undertaken in 2009, 2010 and 2012 by Geoscience Australia, the Australian Institute of Marine Science and the Museum and Art Gallery of the Northern Territory as part of the Australian Government's Offshore Energy Security Initiative and the National Environmental Research Program Marine Biodiversity Hub. All surveys returned geophysical, biological, geochemical, and sedimentological data. Benthic biota were collected with a benthic sled across a range of geomorphic features (bank, terrace, ridge, plain, valley) identified from high-resolution multibeam sonar. Sponges were then taxonomically identified to 350 species, with the species accumulation curve indicating there may be over 900 sponge species in the region. Sponge assemblages were different between the Van Diemen Rise and Londonderry Rise, as well as between individual banks in the same area, indicating that different suites of species occurred at regional (east-west) and local (between banks) scales. Relationships between sponges and other multibeam-derived variables are more complex and warrant further research. The current study will help: i) facilitate integrated marine management by providing a baseline species inventory; ii) support the listing of carbonate banks of the Timor Sea shelf as a Key Ecological Feature, and; iii) inform future monitoring of marine protected area performance, particularly for areas of complex seabed geomorphology.

Submarine canyons have been recognised as areas of significant ecological and conservation value for their enhanced primary productivity, benthic biomass and biodiversity. In Australia, 753 submarine canyons were mapped on all margins of the continent by the Marine Biodiversity Hub through the Australian Government's National Environmental Research Program. An analysis of canyon geomorphic metrics provided the basis to objectively classify these canyons across a hierarchy of physical characteristics (e.g. volume, depth range, rugosity) separately for shelf-incising and slope-confined canyons (Huang et al., 2014). Here we extend this analysis to include oceanographic variables in presenting a first pass assessment of habitat quality for all canyons on the Australian margin, with a focus on their upper reaches. This study is based on the premise that habitat heterogeneity, productivity and disturbance are the three factors that potentially determine the quality of a canyon habitat. For each factor we derived a range of variables to inform the assessment of habitat quality (see Table). Habitat heterogeneity was measured using a selection of eight geomorphic metrics including canyon volume and rugosity that are considered likely to have a positive relationship with habitat heterogeneity. Canyon productivity was assessed from five variables including: distance to the shelf break as a proxy of nutrient inputs from land and the continental shelf; bottom current speed as an indicator of nutrient supply to benthic epifauna (derived from time-series re-analysis of the BLUElink oceanographic model and in-situ data), and; measures of the probability, frequency and intensity of upwelling (also from BLUElink data). The BLUElink variables have positive relationships with productivity whereas the relationship between distance to shelf and productivity is negative. Benthic disturbance was assessed from the maximum and range of bottom current speeds, and the frequency and intensity of tropical cyclones. According to these relationships, individual canyons were assigned habitat quality scores, first separately for each variable and then aggregated for the three habitat factors. The final scores were obtained by averaging the scores of the three habitat factors. The results show that many submarine canyons on the eastern Australian margin have high habitat quality scores (see Figure). This is interpreted to be mainly due to the influence of the upwelling-favourable East Australian Current which generates high productivity throughout the year. The Albany canyons on the south-western margin also offer high habitat quality for marine species due to complex geometrical and geophysical structures. They also benefit from the upwelling-favourable Flinders Current. In contrast, canyons on the northern and western margins have lower habitat quality. Many of these canyons receive little input from land and continental shelf. In addition, the downwelling- favourable Leeuwin Current, which flows along the western margin of the continent, hampers the supply of deep water nutrients from reaching the upper reaches of canyons, particularly canyon heads that intersect the euphotic zone. Overall, these results provide a framework for targeted studies of canyons aimed at testing and verifying the habitat potential identified here and for establishing monitoring priorities for the ongoing management of canyon ecosystems.

Marine organisms are exposed not only to natural environmental stressors, but also the additional effects of anthropogenic stressors, notably increasing temperatures and reduced pH. Early life stages of marine organisms have been recognised as potentially vulnerable to the stressors associated with climate change and ocean acidification, but identifying patterns across studies, species and a range of response variables is challenging. This study is supported by the Marine Biodiversity Hub through the National Environmental Research Program and identifies knowledge gaps in research on multiple abiotic stressors and early life stage (embryo to larvae), while quantifying interactions based on life history. Temperature was the most common stressor (91% of studies), while the most common combination of stressors was temperature and salinity (66%), followed by temperature and pH (17.5%). All studies were conducted in the laboratory although four studies also undertook field experiments. Synergistic interactions (68% of individual tests) were more common than additive (16%) or antagonistic (16%) interactions. The meta-analysis yielded several key results: 1) Embryos are not more vulnerable to stress than larvae in combined stressor treatments. 2) Sub-lethal responses are not more likely to be affected by stress than lethal responses. 3) Interaction types vary among stressors, phyla, ontogenetic stages, and biological responses. 4) Elevated temperature is generally a greater stressor than ocean acidification, but this depends on ontogenetic stage and phylum. 5) Ocean acidification is a greater stressor for calcifying than non-calcifying larvae. Our findings will assist in monitoring and predicting the health of marine populations and communities by identifying sensitive and robust taxa.

This resource contains geochemistry data for the Oceanic Shoals Commonwealth Marine Reserve (CMR) in the Timor Sea collected by Geoscience Australia during September and October 2012 on RV Solander (survey GA0339/SOL5650). This datset comprises chlorophyll a, b and c and phaeophytin a concentrations from the upper 2 cm of seabed sediments. The Oceanic Shoals Commonwealth Marine Reserve survey was undertaken as an activity within the Australian Government's National Environmental Research Program Marine Biodiversity Hub and was the key component of Research Theme 4 - Regional Biodiversity Discovery to Support Marine Bioregional Plans. Hub partners involved in the survey included the Australian Institute of Marine Science, Geoscience Australia, the University of Western Australia, Museum Victoria and the Museum and Art Gallery of the Northern Territory. Data acquired during the survey included: multibeam sonar bathymetry and acoustic backscatter; sub-bottom acoustic profiles; physical samples of seabed sediments, infauna and epibenthic biota; towed underwater video and still camera observations of seabed habitats; baited video observations of demersal and pelagic fish, and; oceanographic measurements of the water column from CTD (conductivity, temperature, depth) casts and from deployment of sea surface drifters. Further information on the survey is available in the post-survey report published as Geoscience Australia Record 2013/38: Nichol, S.L., Howard, F.J.F., Kool, J., Stowar, M., Bouchet, P., Radke, L., Siwabessy, J., Przeslawski, R., Picard, K., Alvarez de Glasby, B., Colquhoun, J., Letessier, T. & Heyward, A. 2013. Oceanic Shoals Commonwealth Marine Reserve (Timor Sea) Biodiversity Survey: GA0339/SOL5650 - Post Survey Report. Record 2013/38. Geoscience Australia: Canberra. (GEOCAT #76658).

Geoscience Australia undertook a marine survey of the Vlaming Sub-basin in March and April 2012 to provide seabed and shallow geological information to support an assessment of the CO2 storage potential of this sedimentary basin. The survey was undertaken under the Australian Government's National CO2 Infrastructure Plan (NCIP) to help identify sites suitable for the long term storage of CO2 within reasonable distances of major sources of CO2 emissions. The Vlaming Sub-basin is located offshore from Perth, Western Australia, and was previously identified by the Carbon Storage Taskforce (2009) as potentially suitable for CO2 storage. The principal aim of the Vlaming Sub-basin marine survey (GA survey number GA0334) was to look for evidence of fault reactivation and of any past or current gas or fluid seepage at the seabed. The survey also mapped seabed habitats and biota in the areas of interest to provide information on communities and biophysical features that may be associated with seepage. This data package brings together the following datasets which describe biophysical aspects of seafloor sediments: GEOCAT#74276. Underwater video footage from the Vlaming Sub-basin (GA0334). GEOCAT#76463. GA0334 Vlaming sub-basin Species identification of worms from grab. GEOCAT#78540. Vlaming Sub-Basin Marine Environmental Survey (GA-0334/S. Supporter GP 1373) (NCIP Program) - High Resolution Bathymetry grids. GEOCAT# 78550. Seabed environments and shallow geology of the Vlaming sub-basin, Western Australia: Chlorin analyses of seabed sediments. GEOCAT#78551. Seabed environments and shallow geology of the Vlaming sub-basin, Western Australia: Inorganic elements of seabed sediments. GEOCAT#78552. Seabed environments and shallow geology of the Vlaming sub-basin, Western Australia: Bulk organic carbon and nitrogen isotopes and concentrations in seabed sediments. GEOCAT#78553. Seabed environments and shallow geology of the Vlaming sub-basin, Western Australia: Sediment oxygen demand of seabed sediments. GEOCAT#78564. Seabed environments and shallow geology of the Vlaming sub-basin, Western Australia: Chlorophyll a, b and c of seabed sediments. GEOCAT#78565. Seabed environments and shallow geology of the Vlaming sub-basin, Western Australia: %carbonate and specific surface area of seabed sediments. GEOCAT#79176. Seabed environments and shallow geology of the Vlaming sub-basin, Western Australia: Grain size and carbonate concentrations of seabed sediments. GEOCAT#79345. Ecology / Infaunal morphospecies identifications from the Vlaming Sub-basin (GA0334). An account of the field operations is published in: GEOCAT 74626. Nicholas, W. A., Borissova, I., Radke, L., Tran, M., Bernardel, G., Jorgensen, D M., Siwabessy, J., Carroll, A. and Whiteway, T., 2012. Seabed Environments and Shallow Geology of the Vlaming Sub-Basin, Western Australia - Marine data for the Investigation of the Geological Storage of CO2. GA0334 Post-Survey Report. Geoscience Australia, Record 2013/09. A preliminary interpretation of seabed data is provided in: GEOCAT 78846. Nicholas, W. A., Howard, F., Carroll, A., Siwabessy, J., Tran, M., Picard, K., Przeslawski, R. and Radke, L. 2014. Seabed Environments and shallow sub-surface geology of the Vlaming Sub-basin, offshore Perth Basin: summary report on observed and potential seepage, and habitats. Geoscience Australia, Record 2014/XXX. Information on the broader study, evaluating the Vlaming Sub-basin CO2 storage potential and providing details of the suitable storage sites, is available in: GEOCAT 79332. Borissova, I, Lech, M.E., Jorgensen, D.C, Southby, C., Wang, L., Bernardel, G., Nicholas, T., Lescinsky, D.L. and Johnston, S. An integrated study of the CO2 storage potential in the offshore Vlaming Sub-basin. Geoscience Australia, Record 2014/XXX.

This report provides details of activities undertaken by the Australian Institute of Marine Science (AIMS), Geoscience Australia, the University of Western Australia and the Museum and Art Gallery of the Northern Territory during a marine biodiversity survey to the Oceanic Shoals Commonwealth Marine Reserve (Timor Sea) in 2012. The survey was an activity within the Australian Government's National Environmental Research Program Marine Biodiversity Hub and is a key component of Theme 4 - Regional Biodiversity Discovery to Support Marine Bioregional Plans. Data collected during the survey will be used to support research being undertaken in other Themes of the Marine Biodiversity Hub, including the modelling of ecosystem processes for the northern region, and to support the work programs of the Department of Environment.