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The secret hidden in dust: Uncovering the potential to use metabarcoding of dust for provenance determination, and the capacity to utilise existing soil reference databases

<div>The ubiquitous nature of dust, along with localised chemical and biological signatures, makes it an ideal medium for provenance determination in a forensic context. Metabarcoding of dust can yield biological communities unique to the site of interest, similarly, geochemical and mineralogical analyses can uncover elements and minerals within dust than can be matched to a geographic location. Combining these analyses presents multiple lines of evidence as to the origin of collected dust samples. In this work, we investigated whether the time an item spent at a site collecting dust influenced the ability to assign provenance. We then integrated dust metabarcoding of bacterial and fungal communities into a framework amenable to forensic casework, (i.e., using calibrated log-likelihood ratios to predict the origin of dust samples) and assessed whether current soil metabarcoding databases could be utilised to predict dust origin. Furthermore, we tested whether both metabarcoding and geochemical/mineralogical analyses could be conducted on a single sample for situations where sampling is limited. We found both analyses could generate results capable of separating sites from a single swabbed sample and that the duration of time to accumulate dust did not impact site separation. We did find significant variation within sites at different sampling time periods, showing that bacterial and fungal community profiles vary over time and space – but not to the extent that they are non-discriminatory. We successfully modelled soil and dust samples for both bacterial and fungal diversity, developing calibrated log-likelihood ratio plots and used these to predict provenance for dust samples. We found that the temporal variation in community composition influenced our ability to predict dust provenance and recommend reference samples be collected as close to the sampling time as possible. Thus, our framework showed soil metabarcoding databases are capable of being used to predict dust provenance but the temporal variation in metabarcoded communities will need to be addressed to improve provenance estimates.&nbsp;</div>

<b>Citation:</b>

Nicole R. Foster, Duncan Taylor, Jurian Hoogewerff, Michael G. Aberle, Patrice de Caritat, Paul Roffey, Robert Edwards, Arif Malik, Michelle Waycott and Jennifer M. Young, The secret hidden in dust: Uncovering the potential to use biological and chemical properties of the airborne soil fraction to assign provenance and integrating this into forensic casework, <i>Forensic Science International: Genetics,</i> (2023)

doi: https://doi.org/10.1016/j.fsigen.2023.102931

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Identification info

Date (Creation)
2022-09-22T16:00:00
Date (Publication)
2023-08-30T21:44:00
Citation identifier
Geoscience Australia Persistent Identifier/https://pid.geoscience.gov.au/dataset/ga/147253

Cited responsible party
Role Organisation / Individual Name Details
Author

Foster, N.R.

External Contact
Author

Taylor, D.

External Contact
Author

Hoogewerff, J.

External Contact
Author

Aberle, M.G.

External Contact
Author

de Caritat, P.

Internal Contact
Author

Roffey, P.

External Contact
Author

Edwards, R.

External Contact
Author

Malik, A.

External Contact
Author

Waycott, M.

External Contact
Author

Young, J.

External Contact
Publisher

Elsevier B.V.

External Contact
Name

Forensic Science International: Genetics

Purpose

Publication in scientific journal (Forensic Science International-Genetics)

Status
Completed
Point of contact
Role Organisation / Individual Name Details
Resource provider

Minerals, Energy and Groundwater Division

External Contact
Point of contact

Commonwealth of Australia (Geoscience Australia)

Voice
Point of contact

Main, P.

MEG Internal Contact
Spatial representation type
Topic category
  • Geoscientific information

Extent

N
S
E
W


Maintenance and update frequency
As needed

Resource format

Title

Product data repository: Various Formats

Website

Data Store directory containing the digital product files

Data Store directory containing one or more files, possibly in a variety of formats, accessible to Geoscience Australia staff only for internal purposes

Keywords
  • soil

Keywords
  • dust

Keywords
  • forensic

Keywords
  • provenance

Keywords
  • intelligence

theme.ANZRC Fields of Research.rdf
  • Soil sciences not elsewhere classified

  • Inorganic geochemistry

  • Mineralogy and crystallography

  • Environmental geography

Keywords
  • Published_External

Resource constraints

Title

Creative Commons Attribution 4.0 International Licence

Alternate title

CC-BY

Edition

4.0

Website

http://creativecommons.org/licenses/

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Use constraints
License
Other constraints

(c) 2023 The Author(s)

Resource constraints

Title

Australian Government Security Classification System

Edition date
2018-11-01T00:00:00
Website

https://www.protectivesecurity.gov.au/Pages/default.aspx

Classification
Unclassified
Classification system

Australian Government Security Classification System

Associated resource

Association Type
Was informed by
Title

Forensic soil provenancing in an urban/suburban setting: a sequential multivariate approach

Citation identifier
144564

Citation identifier
77ec218b-b525-4d73-a5a4-58ef070a653b

Website

https://pid.geoscience.gov.au/dataset/ga/144564

Link to eCat metadata record landing page

Associated resource

Association Type
Was informed by
Title

Forensic soil provenancing in an urban/suburban setting: a simultaneous multivariate approach

Citation identifier
145461

Citation identifier
6d6d100c-6b53-4b78-98e1-56b36b74b4cd

Website

https://pid.geoscience.gov.au/dataset/ga/145461

Link to eCat metadata record landing page

Associated resource

Association Type
Had derivation
Title

The utility of dust as a forensic provenancing material. Exploring collection methods and detection limits for environmental DNA, elemental and mineralogical analyses of dust samples

Citation identifier
147184

Citation identifier
6de1e7af-f0a4-4682-9baa-1853282a406c

Website

https://pid.geoscience.gov.au/dataset/ga/147184

Link to eCat metadata record landing page

Language
English
Character encoding
UTF8

Distribution Information

Distributor contact
Role Organisation / Individual Name Details
Distributor

Commonwealth of Australia (Geoscience Australia)

Voice facsimile
OnLine resource

Link to Journal

Link to Journal

Distribution format

Resource lineage

Statement

<div>eCat 147184</div>

Metadata constraints

Title

Australian Government Security Classification System

Edition date
2018-11-01T00:00:00
Website

https://www.protectivesecurity.gov.au/Pages/default.aspx

Classification
Unclassified

Metadata

Metadata identifier
urn:uuid/bf38894a-5e27-4003-b1a5-b2a52609f907

Title

GeoNetwork UUID

Language
English
Character encoding
UTF8
Contact
Role Organisation / Individual Name Details
Point of contact

Commonwealth of Australia (Geoscience Australia)

Voice
Point of contact

Main, P.

MEG Internal Contact

Type of resource

Resource scope
Document
Name

Journal Article / Conference Paper

Alternative metadata reference

Title

Geoscience Australia - short identifier for metadata record with

uuid

Citation identifier
eCatId/147253

Metadata linkage

https://ecat.ga.gov.au/geonetwork/srv/eng/catalog.search#/metadata/bf38894a-5e27-4003-b1a5-b2a52609f907

Date info (Creation)
2022-11-07T03:20:14
Date info (Revision)
2022-11-07T03:20:14

Metadata standard

Title

AU/NZS ISO 19115-1:2014

Metadata standard

Title

ISO 19115-1:2014

Metadata standard

Title

ISO 19115-3

Title

Geoscience Australia Community Metadata Profile of ISO 19115-1:2014

Edition

Version 2.0, September 2018

Citation identifier
https://pid.geoscience.gov.au/dataset/ga/122551

 
 

Spatial extent

N
S
E
W


Keywords

theme.ANZRC Fields of Research.rdf
Environmental geography Inorganic geochemistry Mineralogy and crystallography Soil sciences not elsewhere classified

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