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The Neoproterozoic–Paleozoic Officer Basin, located in South Australia and Western Australia, remains a frontier basin for energy exploration with significant uncertainty due to a paucity of data. As part of Geoscience Australia’s Exploring for the Future (EFTF) program, the objective of this study is to derive the petrophysical properties and characterise potential reservoirs in the Neoproterozoic–Cambrian sedimentary succession in the Officer Basin through laboratory testing, and well log interpretation using both conventional and neural network methods. Laboratory measurements of forty-one legacy core samples provide the relationships between gas permeability, Klinkenberg corrected permeability, and nano-scale permeability, as well as grain density, effective and total porosity for various rock types. Conventional log interpretation generates the volume fraction of shale, effective and total porosity from gamma ray and lithology logs. Self-organising map (SOM) was used to cluster the well log data to generate petrophysical group/class index and probability profiles for different classes. Neural network technology was employed to approximate porosity and permeability from logs, conventional interpretation results and class index from SOM modelling. The Neoproterozoic-Cambrian successions have the potential to host both conventional and tight hydrocarbon reservoirs. Neoproterozoic successions are demonstrated to host mainly tight reservoirs with the range in average porosity and geometric mean permeability of 4.77%-6.39% and 0.00087-0.01307 mD, respectively, in the different sequences. The range in average porosity and geometric mean permeability of the potential Cambrian conventional reservoirs is 14.54%-26.38% and 0.341-103.68 mD, respectively. The Neoproterozoic shales have favourable sealing capacities. This work updates the knowledge of rock properties to further the evaluation of the resource potential of the Officer Basin. Published in The APPEA Journal 2022 <b>Citation:</b> Wang Liuqi, Bailey Adam H. E., Carr Lidena K., Edwards Dianne S., Khider Kamal, Anderson Jade, Boreham Christopher J., Southby Chris, Dewhurst David N., Esteban Lionel, Munday Stuart, Henson Paul A. (2022) Petrophysical characterisation of the Neoproterozoic and Cambrian successions in the Officer Basin. <i>The APPEA Journal</i><b> 62</b>, 381-399. https://doi.org/10.1071/AJ21076